AUTONOMOUS PROTEIN ENGINEERING

Building an Autonomous Protein Engineering System

ZerothBIO builds experimentally measured functional protein data for protein AI through ProPEL.

Before the First, There’s Zeroth.

ProPELPROtein AI & Precise Experimental Labs
ProPEL closed-loop protein engineering workflow connecting Protein AI with Design, Build, Test and Learn around experimentally measured functional protein data.

WHY ZEROTHBIO

Protein AI needs measured function.

Protein AI can generate more candidates, but development still depends on experimentally measured function. ZerothBIO connects protein design, cell-free production, functional measurement and learning through ProPEL to generate data that can guide the next experiment.

01

Measured

Experimental results are recorded with the conditions needed to interpret them.

02

Functional

The focus is not structure alone, but experimentally observed differences in protein function.

03

Repeatable

Design, experiment and learning are organized as a repeatable operating loop.

04

Learnable

Measured results can become inputs for the next experiment or model-learning step.

MEASURED FUNCTIONAL PROTEIN DATA

From experiments to learnable function.

ZerothBIO’s data narrative starts with experimentally measured protein function and the context required to interpret each result.

DATA POINT ANATOMYMEASURED
Variant / SequenceWhat was tested
ConditionUnder what condition
FunctionWhat was measured
EvidenceHow the result is supported
Learning LinkHow it informs the next decision

TECHNOLOGY

Enabling layers for measured function.

DESIGN

Protein Design

Define candidate hypotheses and search space for experimental evaluation.

BUILD

Combinatorial DNA Library

Create experimental variant libraries that connect design space to wet-lab testing.

BUILD

Cell-Free Protein Synthesis

Produce proteins without a cell-culture step as part of the experimental build workflow.

TEST / LEARN

Functional Measurement & Learning

Measure protein outputs under defined conditions and connect results to the next decision.

COMMERCIAL ENTRY

Cell-Free Protein Synthesis for protein experimentation.

CFPS is ZerothBIO’s commercial entry product for faster protein experimentation.

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

CAT. NO. C1110

ZerothBIO Cell-Free Protein Synthesis Kit

100 rxn

APPROVED BASIS50 µL standard reaction
CAT. NO. C1120

ZerothBIO Cell-Free Protein Synthesis Kit

200 rxn

APPROVED BASIS50 µL standard reaction

Please reference Cat. No. C1110 or C1120 in your inquiry.

TECHNICAL DOCUMENTS

Product Literature & Safety Documents

Download the current product brochure, manual and material safety documents for the ZerothBIO Cell-Free Protein Synthesis Kit.

MANUALPDF

ZerothBIO Cell-Free Protein Synthesis Kit

Product overview, kit contents, specifications and experimental procedure.

Download Manual
MSDSPDF

2x Master Mix

Material Safety Data Sheet for the ZerothBIO 2x Master Mix component.

Download MSDS
MSDSPDF

E. coli Extract

Material Safety Data Sheet for the ZerothBIO E. coli Extract component.

Download MSDS

Product literature and technical documents are provided for research-use product information, operation and safety reference. Always use the latest controlled version supplied by ZerothBIO.

COLLABORATION

Build better protein experiments together.

01

Protein Engineering

Discuss candidate generation, experimental evaluation and iterative protein engineering workflows.

02

Functional Data Generation

Discuss experiments designed to produce measured protein-function data under defined conditions.

03

Platform Collaboration

Explore how ProPEL workflows can connect design, experimentation, measurement and learning for a defined project.

ABOUT ZEROTHBIO

Make Protein Function Learnable.

ZerothBIO builds experimentally measured functional protein data for protein AI. Through ProPEL, the company connects protein design, experimental execution, functional measurement and learning in an autonomous protein engineering architecture.

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